BioScience Trends. 2012;6(6):313-324. (DOI: 10.5582/bst.2012.v6.6.313)

Effect of mild hypothermia on breast cancer cells adhesion and migration.

Zhang XM, Lv YG, Chen GB, Zou Y, Lin CW, Yang L, Guo P, Lin MP


To explore the effect of mild hypothermia (35ºC) on breast cancer cells adhesion to vascular endothelial cells, a parallel plant flow chamber was used to observe the adhesion of human breast cancer cells MDA-MB-231 to endothelial cells Eahy926 under physiological flow at 35ºC and 37ºC, as well as the role of intercellular adhesion molecule 1 (ICAM-1) in this process. Further, the effect of mild hypothermia (35ºC) on migration of MDA-MB-231 was also studied. Our results show that mild hypothermia can inhibit the adhesion of tumor cells to endothelial cells and ICAM-1 plays an important role in this process. However, mild hypothermia inhibits breast cancer cell adhesion in a way independent on the change of ICAM-1 expression under our experimental conditions. Mild hypothermia can weaken the chemotaxis of breast cancer cells while it has no obvious effect on unidirectonal migration capacity. These results suggest that mild hypothermia could be used as a potentially adjunct treatment combined with surgery to decrease tumor cell adhesion and migration.

KEYWORDS: Mild hypothermia, breast cancer, cell adhesion, cell migration, intercellular adhesion molecule 1, flow chamber

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