BioScience Trends. 2017;11(3):363-365. (DOI: 10.5582/bst.2017.01037)

Potential proteins targeted by let-7f-5p in HeLa cells.

Wang Y, Chen XJ, Zhang Y, Song JD


MicroRNAs are a class of small, endogenous, non-coding RNAs mediating posttranscriptional gene silencing. The current authors hypothesized that let-7f-5p is likely involved in cell invasion and proliferation by regulating the expression of target genes. The current study combined let-7f-5p with iTRAQ to assess its effect on gene expression in HeLa cells. Results indicated that 164 proteins were expressed at different levels in HeLa cells overexpressing let- 7f-5p and negative controls and that 172 proteins were expressed at different levels in let-7f- 5p-silenced HeLa cells and negative controls. Results indicated that let-7f-5p may suppress insulin-like growth factor 2 mRNA binding protein 1 (IGF2BP1) in HeLa cells.

KEYWORDS: Proteomic analysis, let-7f-5p, IGF2BP1, HeLa cells

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